| This
system is a laser scanning confocal microscope with multiphoton capability.
It excites traditional fluorescent dyes (which normally require visible
wavelengths) with rapid, intense pulses of infrared laser light. As
a result of this design, multiphoton microscopy is far less toxic
to living samples and can see much deeper into cells, tissues, organs,
and even living organisms. Our instrument is optimized for simultaneous
imaging and electrophysiological studies. |
- Zeiss
Axioskop 2 FS MOT upright microscope
- Fixed
stage with motorized x, y, and z control
- Confocal
imaging with two visible-light lasers:
- 25
mW argon laser exciting at 458, 488, and 514 nm
- 1
mW helium-neon laser exciting at 543 nm
- Multiphoton
imaging with a Mira 900-F femtosecond-pulsed titanium-sapphire
laser (typical output power 800 mW) tunable from 700-900 nm
- Filters
for blue, green, yellow, and red emitting fluorescent labels
- Phase
or differential interference contrast (Nomarski) imaging, including
infrared DIC
- Although
not a confocal image, this can serve as a convenient, superimposable
companion to the fluorescence image and can be used to locate
non-fluorescent structures
- 10x
and 40x objectives; the 40x can be used with or without a coverslip
and is designed for electrophysiological applications. Users may
also bring other suitable Zeiss objectives.
- Pentium
PC computer running Windows NT 4.0 with Zeiss 3D for LSM software
for analysis and 3D reconstruction
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