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Time                    Slide Title:

00:01:25.8     Nucleus accumbens & midbrain tegmentum
00:01:42.0     Dopamine transporter action
00:02:39.1     Action of Opiate Receptors and Dopamine Reuptake Transporter
00:04:21.3     Rhesus monkey: Heroin Self-administration pre immunization
00:05:06.1     Rhesus monkey: Heroin Self-administration post immunization
00:05:50.1     Anti-opiate antibodies in the Rhesus monkey
00:06:51.3     11C (-) cocaine & 11C (+) cocaine
00:08:10.3     11C (-) cocaine & 11C (+) cocaine concentration in blood after injection
00:08:33.8     11C (-) cocaine & 11C (+) cocaine percent hydrolysis
00:08:52.3     Metabolism of 11C (-) cocaine & 11C (+) cocaine by butrylcholinesterase
00:10:25.2     Catalyst effect on reaction free energy
00:12:48.2     Hydrolysis of Cocaine: Mechanism
00:14:13.0     The three transition-state analogs
00:14:38.6     Analysis - possible reaction from TSA to ecgonine
00:15:14.0     Synthesis of TSA 1
00:15:19.6     Procedure for making catalytic antibodies
00:16:15.7     Reaction to identify catalytic antibodies
00:17:12.1     Release of benzoic acid
00:17:56.7     Km & Kcat for 3B9 & 6A12 shows saturable hydrolysis
00:18:16.7     
00:18:35.5     Synthesis of TSA 2
00:18:43:6     Synthesis of TSA 3
00:19:11.8     Kinetic parameters for the hydrolysis of 3H Cocaine by Mabs
00:19:53.9     Optimization of Cocaine Esterase Activity
00:21:10.1     Strategies for the design of Transition-State Analogs for Cocaine Hydrolysis
00:22:27.7     Rate constants of Ester Hydrolysis for Cocaine & Homologs
00:23:23.1     Hydrolysis of Cocaine & Pseudococaine: pH dependence
00:24:12.6     Mechanism for the Non-enymatic Hydrolysis of the Cocaine Methyl Ester
00:24:51.7     Hapten for Substrate-assisted Benzoate Hydrolysis of Cocaine: Design
00:24:55.4     Optimization of Cocaine Esterase Activity
00:25:17.2     Plot of log(Km/K_TSA4 versus log(K_cat/K_uncat) for catalytic antibodies generated by TSA 1, 2, & 3.
00:26:07.3     Competitive Inhibition Enzyme Immunoassay of Catalytic Mabs.
00:26:55.5     Deduced Amino Acid Sequences of Catalytic Antibody Light & heavy chain CDR's
00:27:50.5     Monoclonal antibodies 15A10, 19G8, 9A3
00:28:02.4     Overdose model: survival rates at overdose v. dose of 15A10
00:29:40.4     Dose required for seizure & death (saline, cocaine, cocaine + Mab 15A10)
00:30:15.8     Mab 15A10 increases cocaine degradation in the rat
00:30:52.2     EME and cocaine levels at death
00:31:31.5     Self-administration of cocaine & saline
00:32:38.0     Self-administration of cocaine alone, saline, & cocaine after Mab 15A10

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